Categories
- Adult stem cell
Adipose Stem Cell
Cancer stem cell
Cell transplantation
Endothelial stem/progenitor cell
Epithelial stem cell
Glial Stem Cell
Hematopoietic stem cell
Intestinal stem cell
Maintenance and differentiation
Mesenchymal stem cell
Muscle stem cell
Neural stem cell
Stromal cell
+ Embryonic stem cell
+ Germ cell
Organoid culture
+ Pluripotent stem cell
Protocols in Past Issues

Mouse Müller Cell Isolation and Culture

XL Xiao Liu
LT Luosheng Tang
YL Yongqing Liu
13016 Views
Aug 5, 2017
Müller cells are the major supportive and protective glial cells across the retina. Unlike in fish, they have lost the capacity to regenerate the retina in mammals. But, mammalian Müller cells still retain certain retinal stem cell properties with various degree of self-renewal and differentiation potentials, and thereby held a merit in cell-based therapies for treating retinal degeneration diseases. In our laboratory, we use an enzymatic procedure to isolate, purify, and culture mouse Müller cells.